This module will explore advanced biomedical techniques and their applications in molecular diagnostics and related areas. Basic principles in cloning will be covered. Introduction to gene manipulation and genetic engineering including ethical aspects. PCR Technology. Immunofluorescence. Flow cytometry. DNA fingerprinting: applications including in forensics. DNA sequencing. Western Blotting. Northern blot. Southern blot. In situ hybridization, pre-natal diagnosis, mass screening, monoclonal antibodies as probes, detection of microbial diseases and multifactorial diseases. Advantages and disadvantages of diagnostic kits. Further study of diagnostic enzymes. Tumour markers. This module will include demonstrations.
