Introduction to enzyme catalysis and mechanisms of enzyme action. Enzyme inhibition. Further study of the major classes of biomolecules: lipids, amino acids, proteins, carbohydrates, nucleotides and nucleic acids. Principles of metabolism and metabolic regulation. Bioenergetics. ATP cycle. Carbohydrate metabolism. Lipid metabolism. Amino acid metabolism. Biochemistry of cell death. Biochemical aspects of cell signaling. Free radical in cell and organ function.